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KMID : 1005220130280020127
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Journal of Embryo Transfer
2013 Volume.28 No. 2 p.127 ~ p.132
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Effect of Oocyte Maturation Medium, Cytochalasin Treatment and Electric Activation on Embryonic Development after Intracytoplasmic Sperm Injection in Pigs
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Lee Joo-Hyeong
Choi Jung-Hoon
Lee Seung-Tae
Hyun Sang-Hwan
Lee Eun-Song
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Abstract
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The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05
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KEYWORD
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cytochalasin B , electric activation , embryonic development , ICSI , pig
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